How to prepare the sample for XRD analysis? (sample preparation for XRD analysis)


XRD is used for the elemental detection and purity of the test sample. Specific protocol should be followed to prepare sample for XRD analysis which includes a few steps prior to be subjected under x-ray diffraction machine. Minimum requirement of sample to be analyzed through XRD technique is one gram. The sample should be purified prior to analysis. 

Complete procedure to prepare XRD sample is being discussed in detail in this article. Solid crystalline sample is first manually grinded thoroughly to obtain smooth and fine sample in the form of powder. Afterwards, an organic solvent, mostly ethanol is added in small amount and grinned again. The purpose of addition of ethanol during grinding procedure is to lower the surface energy of test sample that can otherwise disrupts not only the formation but also specific position of peaks in XRD pattern. Another edge of using solvent during grinding is that it significantly reduces the mesh size of the particles. The mesh size of particles of a test sample should be lower than 10 micrometer. Grinding is then followed by taking the sample is a sample holder. The sample is spread on a glass slide and packed inside sample container or it can also be distributed on a sticky tape by spraying it on the tape. A small amount of reference standard is usually added in the analyzing product which can assist in elemental identification process. At this stage sample is ready for analysis.

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